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icasp9 ha sequence  (Addgene inc)


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    Structured Review

    Addgene inc icasp9 ha sequence
    Icasp9 Ha Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icasp9+ha+sequence/pMSCV-F-del+Casp9%2EIRES%2EGFP+(Plasmid+%2315567)/pm41819100-326-24-30
    Average 93 stars, based on 39 article reviews
    icasp9 ha sequence - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: The following plasmids were obtained from Addgene: pMSCV-F-del-Casp9-IRES-GFP (David Spencer, Plasmid# 15567), rtTA-N144 (Andrew Yoo, Plasmid# 66810), pXG237 (Martin Kampmann, Plasmid# 141281), pRK5-HA-Ubiquitin-WT (Ted Dawson, Plasmid# 17608), pMDLg/Prre (Plasmid# 12251), pRSV-Rev (Plasmid# 12253), psPAX2 (Plasmid# 12260), and pMD2.G (Plasmid# 12259) from Didier Trono, and pVSV-G (Akitsu Hotta, Plasmid# 138479). .. To construct the EF1α-uORF1-uORF2-iCasp9-HA (uORFsiCasp9-HA) plasmid, the upstream open reading frame (uORF) sequence of ATF4 was amplified from pXG237 (Addgene, Plasmid# 141281), and the iCasp9-HA sequence was amplified from pMSCV-F-del-Casp9-IRES-GFP (Addgene, Plasmid# 15567). .. PCR Neuron 114, 1–19.e1–e14, June 17, 2026 e8 primers were designed with 15-20 bp homologous arms, and the fragments were assembled into the BamHI (NEB, R3136S)/NotI (NEB, R3189S)-digested rtTA-N144 (Addgene, Plasmid# 66810) vector using homologous recombination.

    Plasmid Preparation:

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: The following plasmids were obtained from Addgene: pMSCV-F-del-Casp9-IRES-GFP (David Spencer, Plasmid# 15567), rtTA-N144 (Andrew Yoo, Plasmid# 66810), pXG237 (Martin Kampmann, Plasmid# 141281), pRK5-HA-Ubiquitin-WT (Ted Dawson, Plasmid# 17608), pMDLg/Prre (Plasmid# 12251), pRSV-Rev (Plasmid# 12253), psPAX2 (Plasmid# 12260), and pMD2.G (Plasmid# 12259) from Didier Trono, and pVSV-G (Akitsu Hotta, Plasmid# 138479). .. To construct the EF1α-uORF1-uORF2-iCasp9-HA (uORFsiCasp9-HA) plasmid, the upstream open reading frame (uORF) sequence of ATF4 was amplified from pXG237 (Addgene, Plasmid# 141281), and the iCasp9-HA sequence was amplified from pMSCV-F-del-Casp9-IRES-GFP (Addgene, Plasmid# 15567). .. PCR Neuron 114, 1–19.e1–e14, June 17, 2026 e8 primers were designed with 15-20 bp homologous arms, and the fragments were assembled into the BamHI (NEB, R3136S)/NotI (NEB, R3189S)-digested rtTA-N144 (Addgene, Plasmid# 66810) vector using homologous recombination.

    Sequencing:

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: The following plasmids were obtained from Addgene: pMSCV-F-del-Casp9-IRES-GFP (David Spencer, Plasmid# 15567), rtTA-N144 (Andrew Yoo, Plasmid# 66810), pXG237 (Martin Kampmann, Plasmid# 141281), pRK5-HA-Ubiquitin-WT (Ted Dawson, Plasmid# 17608), pMDLg/Prre (Plasmid# 12251), pRSV-Rev (Plasmid# 12253), psPAX2 (Plasmid# 12260), and pMD2.G (Plasmid# 12259) from Didier Trono, and pVSV-G (Akitsu Hotta, Plasmid# 138479). .. To construct the EF1α-uORF1-uORF2-iCasp9-HA (uORFsiCasp9-HA) plasmid, the upstream open reading frame (uORF) sequence of ATF4 was amplified from pXG237 (Addgene, Plasmid# 141281), and the iCasp9-HA sequence was amplified from pMSCV-F-del-Casp9-IRES-GFP (Addgene, Plasmid# 15567). .. PCR Neuron 114, 1–19.e1–e14, June 17, 2026 e8 primers were designed with 15-20 bp homologous arms, and the fragments were assembled into the BamHI (NEB, R3136S)/NotI (NEB, R3189S)-digested rtTA-N144 (Addgene, Plasmid# 66810) vector using homologous recombination.

    Amplification:

    Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
    Article Snippet: The following plasmids were obtained from Addgene: pMSCV-F-del-Casp9-IRES-GFP (David Spencer, Plasmid# 15567), rtTA-N144 (Andrew Yoo, Plasmid# 66810), pXG237 (Martin Kampmann, Plasmid# 141281), pRK5-HA-Ubiquitin-WT (Ted Dawson, Plasmid# 17608), pMDLg/Prre (Plasmid# 12251), pRSV-Rev (Plasmid# 12253), psPAX2 (Plasmid# 12260), and pMD2.G (Plasmid# 12259) from Didier Trono, and pVSV-G (Akitsu Hotta, Plasmid# 138479). .. To construct the EF1α-uORF1-uORF2-iCasp9-HA (uORFsiCasp9-HA) plasmid, the upstream open reading frame (uORF) sequence of ATF4 was amplified from pXG237 (Addgene, Plasmid# 141281), and the iCasp9-HA sequence was amplified from pMSCV-F-del-Casp9-IRES-GFP (Addgene, Plasmid# 15567). .. PCR Neuron 114, 1–19.e1–e14, June 17, 2026 e8 primers were designed with 15-20 bp homologous arms, and the fragments were assembled into the BamHI (NEB, R3136S)/NotI (NEB, R3189S)-digested rtTA-N144 (Addgene, Plasmid# 66810) vector using homologous recombination.



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    Addgene inc icasp9 ha sequence
    Icasp9 Ha Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icasp9+ha+sequence/pMSCV-F-del+Casp9%2EIRES%2EGFP+(Plasmid+%2315567)/pm41819100-326-24-30
    Average 93 stars, based on 1 article reviews
    icasp9 ha sequence - by Bioz Stars, 2026-09
    93/100 stars
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